Anti-Histamine H3 Receptor (HRH3) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5N1

Core Product Specifications and Parameters

Biological Background: Histamine H3 Receptor (HRH3) Function and Localization

• Histamine H3 receptor (HRH3) is a G protein-coupled receptor (GPCR) that primarily mediates histamine signaling in the central and peripheral nervous systems.
• Its signaling occurs through inhibition of adenylate cyclase and the receptor exhibits high constitutive activity (spontaneous activity in the absence of agonist).
• Isoform 3 of HRH3 does not couple to adenylate cyclase inhibition or intracellular calcium mobilization upon agonist stimulation.
• HRH3 is localized to the cell membrane.
• The receptor is predominantly expressed in the CNS, with highest levels in the thalamus and caudate nucleus. Multiple isoforms (3,5,6,7) show region-specific expression patterns in the brain.
• Key structural features include seven transmembrane helices, disulfide bonds, and post-translational modifications such as glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a band near 49 kDa corresponding to HRH3; use appropriate loading controls and validate with peptide blocking if needed.
• In immunofluorescence/ICC, fixation and permeabilization protocols may need optimization due to the receptor's membrane localization.
• Due to high sequence homology among human, mouse, and rat HRH3, confirm species-specific reactivity using lysates from each species.
• Consider testing multiple antibody dilutions and incubation conditions, as GPCR antibodies often require empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this HRH3 rabbit polyclonal antibody as part of a comprehensive portfolio for neuroscience and GPCR research. We provide one-stop sourcing of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement to support your IVD development from concept to clinic.