Anti-Histamine H2 Receptor Polyclonal Antibody for WB and ELISA - P25021

Core Product Specifications and Parameters

Biological Background: Histamine H2 Receptor Function and Localization

• Histamine H2 receptor (HRH2, also known as H2R, HH2R, or gastric receptor I) is a G protein-coupled receptor encoded by the HRH2 gene in humans and exists in several alternatively spliced isoforms.
• Upon binding histamine, HRH2 predominantly couples to G(s) alpha proteins, activating adenylate cyclase and increasing intracellular cAMP levels, which stimulates gastric parietal cells to secrete hydrochloric acid (PubMed:38647423, PubMed:39333117). Related references: PMID:38647423 PMID:39333117
• HRH2 is also expressed in the heart and central nervous system, where it may contribute to cardiac stimulation and modulate neurotransmitter release.
• The receptor localizes to the cell membrane, consistent with its role as a transmembrane signaling protein.
• Key post-translational modifications include glycosylation, palmitoylation, and the formation of disulfide bonds, as annotated in UniProt keywords.
• The molecular weight of the mature protein is approximately 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 150-250 of human HRH2. Consider sequence alignment with mouse and rat HRH2 to confirm epitope conservation across species when planning cross-species experiments.
• This antibody has been listed for Western blot (WB) and ELISA. For WB, a band at ~40 kDa is anticipated. Validate the antibody in your sample system, as detection efficiency can vary with sample preparation and expression levels.
• When using ELISA, titrate the antibody and include appropriate controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a one-stop sourcing platform for IVD raw materials. As a supplier of this HRH2 polyclonal antibody, we support the development of assays targeting the histamine H2 receptor, a key player in gastric acid secretion and GPCR signaling. Our portfolio includes validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, ensuring reliable procurement for your diagnostic or research workflows.