Anti-HIRIP3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BW71

Core Product Specifications and Parameters

Biological Background: HIRIP3 Function and Localization

• HIRIP3 (HIRA-interacting protein 3) serves as a histone chaperone that specifically facilitates the deposition of H2A-H2B histone complexes onto chromatin.
• The protein is predominantly localized in the nucleus, consistent with its role in chromatin assembly and regulation.
• HIRIP3 undergoes alternative splicing, giving rise at least two isoforms: isoform 1 predominates in skeletal muscle, while isoform 2 is more abundant in liver and heart.
• Post-translational modifications include phosphorylation, as identified by proteomics, suggesting potential regulatory mechanisms.
• Functional keywords associated with HIRIP3 include chaperone, chromatin regulator, alternative splicing, nucleus, and phosphoprotein, underscoring its role in epigenetic control.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 417-556 of human HIRIP3 (NP_003600.2), providing specificity for detection; consider sequence alignment when applying to other species.
• For Western blotting, a band near 62 kDa is expected; validate with appropriate positive and negative controls.
• The antibody cross-reacts with human, mouse, and rat samples; for untested species, check epitope conservation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions for your specific tissue or cell type.
• For ELISA applications, empirical titration of both capture and detection antibodies is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and expert consulting. This anti-HIRIP3 antibody is a valuable tool for chromatin biology and epigenetic research. Beyond single reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing — supporting your projects from concept to clinic.