Anti-HIRA Polyclonal Antibody for WB and ELISA - P54198

Core Product Specifications and Parameters

Biological Background: HIRA Function and Localization

• HIRA is a multi-domain protein containing WD repeats, identified as a TUP1-like enhancer of split protein 1, involved in chromatin regulation and transcriptional repression.
• It cooperates with ASF1A to facilitate replication-independent chromatin assembly, ensuring proper nucleosome deposition outside of S-phase.
• HIRA is essential for periodic repression of histone gene transcription during the cell cycle, linking chromatin assembly with cell cycle progression.
• The protein directs formation of senescence-associated heterochromatin foci (SAHF), contributing to permanent cell cycle exit in senescent cells.
• Subcellularly, HIRA localizes predominantly to the nucleus and can be found in PML bodies, consistent with its role in nuclear processes.
• Tissue expression analysis shows high levels in kidney, pancreas, and skeletal muscle, with moderate to low expression in brain, heart, liver, lung, and placenta.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, which may regulate its activity in chromatin dynamics.
• Alternative splicing produces multiple isoforms, potentially modulating HIRA's functional repertoire in different cellular contexts.

Experimental Guidance and Technical Tips

• Since the immunogen covers a central region (aa 250-550), this antibody may detect multiple HIRA isoforms; validate banding patterns in your experimental system.
• For Western blot, expect a major band around 112 kDa; include appropriate positive controls (e.g., HeLa or kidney cell lysates) and molecular weight markers.
• Cross-reactivity with human and mouse is indicated; verify performance in your target species if other than these two.
• In ELISA applications, consider using this polyclonal antibody as both capture and detection (if paired with appropriate secondaries) for sandwich assays; optimize coating and blocking conditions.

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CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-HIRA, bulk ancillary reagents, and rare target sourcing. For researchers and assay developers focusing on chromatin biology, epigenetics, or cellular senescence, we support your project from early development through clinical application with reliable materials and technical expertise.