Anti-HIRA Monoclonal Antibody for WB, ELISA - P54198

Core Product Specifications and Parameters

Biological Background: HIRA Function and Localization

• Protein HIRA, also known as TUP1-like enhancer of split protein 1, is encoded by the HIRA gene (also known as DGCR1, HIR, TUPLE1). It belongs to the WD repeat protein family and functions as a chromatin regulator.
• Cooperates with ASF1A to facilitate replication-independent chromatin assembly, depositing histones onto DNA in a DNA-synthesis-independent manner.
• Essential for the periodic repression of histone gene transcription during the cell cycle, ensuring proper temporal control.
• Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
• Subcellular localization: predominantly nuclear, with enrichment in PML bodies.
• Tissue specificity: highly expressed in kidney, pancreas, and skeletal muscle; lower expression in brain, heart, liver, lung, and placenta.
• Post-translational modifications: phosphorylation and ubiquitin-like conjugation have been identified, which may regulate its activity.
• Alternative splicing generates multiple transcript variants, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 550-675 of human HIRA. This region is specific to HIRA and is not expected to cross-react with other WD repeat proteins.
• For Western blotting, the predicted molecular weight of HIRA is approximately 112 kDa; consider using appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In ELISA, the antibody may be used for direct detection of recombinant or endogenous HIRA; validate in your sample system with appropriate controls.
• Since this is a rabbit monoclonal antibody, it offers high specificity and batch-to-batch consistency; consider using it in multiplexed detection protocols if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your entire diagnostic development workflow. This anti-HIRA monoclonal antibody is particularly suited for research into chromatin dynamics, cell cycle regulation, and senescence. Whether you need consistent supplies for kit manufacturing or custom reagent sourcing, CamelBio bridges the gap from concept to clinic.