Anti-Hippocalcin (HPCA) Rabbit Monoclonal Antibody for WB, ELISA - P84074

Core Product Specifications and Parameters

Biological Background: Hippocalcin (HPCA) Function and Localization

• Neuron-specific calcium-binding protein hippocalcin (HPCA), also known as calcium-binding protein BDR-2, encoded by the HPCA gene in humans.
• Functions as a calcium sensor, potentially regulating voltage-dependent calcium channels. Related references: PMID:28398555
• May modulate cyclic-nucleotide-mediated signaling by affecting adenylate and guanylate cyclase activities.
• Primarily localized in the cytoplasm and cytosol, and can associate with the membrane.
• Contains N-terminal myristoylation, a lipid modification that likely facilitates membrane binding.
• Predominantly expressed in the brain, consistent with its role in neuronal function.
• Belongs to the recoverin family of neuronal calcium sensor proteins, characterized by EF-hand domains.
• Associated with dystonia (DYT2) based on disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–100); consider that endogenous myristoylation may influence antibody binding in native samples.
• For Western blot, expect a band around 22 kDa in brain tissue lysates; validate in your specific sample system.
• Cross-reactivity has been confirmed for human, mouse, and rat; for other species, verify sequence homology before use.
• In ELISA, pre-coating with the immunizing peptide or recombinant protein may serve as a positive control.
• Monoclonal antibody ensures lot-to-lot consistency; always titrate for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. This anti-Hippocalcin antibody supports neuroscience research and assay development targeting calcium-binding proteins. Our supply capabilities include validated antibody pairs, bulk monoclonal antibodies, and custom sourcing for rare targets, helping you accelerate from concept to clinic.