Anti-HIPK2 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9H2X6

Core Product Specifications and Parameters

Biological Background: HIPK2 Function and Localization

• HIPK2 is a serine/threonine kinase that phosphorylates key regulators such as p53/TP53, CREB1, and CTNNB1, inhibiting cell growth and promoting apoptosis.
• Acts as a transcriptional corepressor for factors like SMAD1 and POU4F1, and modulates Wnt/beta-catenin signaling by promoting proteasomal degradation of CTNNB1 and MYB.
• In response to DNA damage, HIPK2 phosphorylates DAZAP2, localizing it to the nucleus and preventing SIAH1-mediated ubiquitination of HIPK2. Related references: PMID:33591310
• Subcellular localization includes nucleus, PML bodies, cytoplasm, and stress granules, reflecting its dynamic role in signaling.
• Highly expressed in heart, muscle, and kidney; down-regulated in certain thyroid and breast tumors.
• Alternatively spliced isoforms produce proteins of ~101–131 kDa, consistent with observed multiple bands in western blot.
• Key post-translational modifications include phosphorylation and ubiquitination; implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 100-200 of human HIPK2; verify reactivity in target species and tissue.
• For Western blot, multiple bands around 101–131 kDa may be observed due to alternative splicing; include appropriate controls.
• In immunofluorescence, anticipate nuclear and possibly cytoplasmic localization; optimize fixation and permeabilization conditions.
• For ELISA, consider pairing with a detection antibody targeting a distinct epitope; validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality IVD raw materials, from concept to clinic. This anti-HIPK2 monoclonal antibody is part of our comprehensive portfolio for oncology and cell signaling applications. We also offer validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate your assay development.