Anti-HIP1 Polyclonal Antibody for WB and ELISA - O00291

Core Product Specifications and Parameters

Biological Background: HIP1 Function and Localization

• HIP1 (Huntingtin-interacting protein 1), also known as HIP-I, SHON, and ILWEQ, is a multi-functional scaffold protein involved in endocytosis, transcription, and apoptosis.
• Core Function: HIP1 plays a critical role in clathrin-mediated endocytosis and intracellular trafficking (PubMed:11532990, PubMed:11577110, PubMed:11889126). Related references: PMID:11532990 PMID:11577110 PMID:11889126
• In the central nervous system, HIP1 regulates AMPA receptor trafficking and presynaptic nerve terminal activity, likely through its endocytic role (By similarity).
• HIP1 enhances androgen receptor (AR)-mediated transcription, linking endocytic machinery to hormonal signalling (PubMed:16027218). Related references: PMID:16027218
• It may act as a proapoptotic factor, inducing cell death via the intrinsic apoptosis pathway (PubMed:11007801). Related references: PMID:11007801
• HIP1 binds 3-phosphoinositides through its ENTH domain, promoting cell survival by stabilizing receptor tyrosine kinases after ligand-induced endocytosis (PubMed:14732715). Related references: PMID:14732715
• It also associates with filamentous actin and may regulate cytoskeletal organization (PubMed:18790740). Related references: PMID:18790740
• Ubiquitously expressed with highest levels in brain, HIP1 is upregulated in prostate and colon cancers, implicating it in tumorigenesis.
• Subcellularly, HIP1 localizes to the cytoplasm, nucleus, and clathrin-coated vesicle membranes, reflecting its diverse functions.

Experimental Guidance and Technical Tips

• The immunogen spans the central region (aa 410-690) of human HIP1, which may detect multiple isoforms generated by alternative splicing.
• For Western blotting, expect a main band at ~116 kDa; perform proper titration to minimize non-specific binding often observed with polyclonal antibodies.
• Validate the antibody in your specific sample type, as HIP1 expression is ubiquitous but varies; enriched in brain and certain cancers.
• For ELISA applications, consider pairing with a matched monoclonal detection antibody or using recombinant HIP1 protein as a reference standard.
• Cross-reactivity with mouse HIP1 allows translational studies, but always confirm sequence homology in the epitope region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and tailored sourcing solutions. This anti-HIP1 polyclonal antibody further strengthens our portfolio for neurodegenerative disease and cancer research tools. From validated antibody pairs and bulk ancillary reagents to rare target procurement, we streamline your supply chain from discovery to clinical application.