Anti-HINT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49773

Core Product Specifications and Parameters

Biological Background: HINT1 Function and Localization

• HINT1 (Histidine triad nucleotide-binding protein 1), also known as Protein kinase C inhibitor 1 (PKCI-1), is a ubiquitously expressed enzyme belonging to the histidine triad (HIT) superfamily.
• It primarily functions as an adenosine 5'-monophosphoramidase, hydrolyzing a variety of substrates including purine nucleotide phosphoramidates (AMP-NH2, AMP-morpholidate, GMP-morpholidate), aminoacyl-AMP conjugates (lysyl-AMP, Met-AMP, His-AMP, Asp-AMP, lysyl-GMP), and fluorogenic tryptamine phosphoramidates. Related references: PMID:15703176 PMID:16835243 PMID:17217311 PMID:17337452 PMID:22329685 PMID:23614568
• It can also convert adenosine/guanosine 5'-O-phosphorothioate to nucleoside 5'-phosphates with H2S release. Related references: PMID:30772266
• Beyond its enzymatic activity, HINT1 acts as a scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and the MITF/CTNNB1 complex, implicating it in Wnt and MITF signaling pathways. Related references: PMID:16014379 PMID:22647378
• It influences apoptosis by modulating p53/TP53 protein levels and p53-mediated apoptosis, and can regulate proteasomal degradation of target proteins through the SCF E3 ubiquitin ligase complex. Related references: PMID:16835243 PMID:19112177
• HINT1 also displays SUMO-specific isopeptidase activity, deconjugating SUMO1 from substrates such as RGS17 and RANGAP1, indicating a role in SUMOylation dynamics. Related references: PMID:31088288
• Subcellularly, it is found in both the cytoplasm and nucleus, consistent with its diverse functions in nucleotide metabolism and transcriptional regulation.
• Mutations in HINT1 are associated with autosomal recessive axonal neuropathy with neuromyotonia, highlighting its clinical relevance in neurological disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human HINT1 protein (amino acids 1-126), suggesting the antibody may recognize the native conformation of the protein in addition to denatured forms.
• Based on its validated reactivity with human, mouse, and rat samples, this antibody is suitable for cross-species studies; verify performance in specific models.
• For Western blot, the predicted molecular weight is ~14kDa; use appropriate gel percentage and loading controls to confirm specificity.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to ensure penetration and preservation of antigenicity, and include appropriate negative controls.
• In ELISA, pair with a matched detection antibody or substrate system; validate the assay with known positive/negative controls to establish sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers investigating HINT1's roles in nucleotide metabolism, transcriptional regulation, and neurological disease, we provide streamlined access to high-quality reagents and custom production services to accelerate assay development and diagnostic innovation.