Anti-HINT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49773

Core Product Specifications and Parameters

Biological Background: HINT1 Function and Localization

• Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the HIT superfamily and is also known as protein kinase C inhibitor 1 (PKCI-1) and adenosine 5'-monophosphoramidase.
• Functions as a hydrolase that catalyzes the hydrolysis of purine nucleotide phosphoramidates, such as adenosine 5'-monophosphoramidate (AMP-NH2), to AMP and ammonia. It also hydrolyzes various aminoacyl-AMP and morpholidate substrates. Related references: PMID:15703176, PMID:16835243, PMID:17217311, PMID:17337452, PMID:22329685, PMID:23614568, PMID:30772266
• Acts as a desumoylating isopeptidase, deconjugating SUMO1 from proteins such as RGS17 and RANGAP1. Related references: PMID:31088288
• Serves as a scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 and MITF-CTNNB1 complexes. Related references: PMID:16014379, PMID:22647378
• Modulates p53/TP53 protein levels and p53-mediated apoptosis. Related references: PMID:16835243
• Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Related references: PMID:19112177
• Localizes to both the cytoplasm and nucleus, consistent with its diverse enzymatic and regulatory roles.
• Ubiquitously expressed across tissues, reflecting its fundamental cellular functions.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length HINT1 protein (amino acids 1–126), suggesting the antibody may recognize linear and conformational epitopes.
• For Western blot, the predicted molecular weight is ~14 kDa. Use appropriate gel percentage and membrane transfer conditions to retain this small protein.
• In IF/ICC, optimize fixation (e.g., 4% PFA versus methanol) and permeabilization, as detection of nuclear and cytoplasmic pools may vary.
• Validate the antibody in the relevant sample system (human, mouse, or rat) using known positive and negative control lysates or cells, as cross-reactivity may differ by application.
• For ELISA, consider direct coating of recombinant HINT1 protein or a sandwich format; confirm the antibody’s reactivity with the coating antigen under your buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing researchers and diagnostic manufacturers with reliable access to validated antibodies, antigens, and ancillary reagents. This polyclonal antibody targeting the nucleotide metabolite regulator HINT1 supports investigations into apoptosis, transcription regulation, and SUMOylation pathways. Beyond single products, CamelBio offers one-stop sourcing for optimized antibody pairs, bulk raw materials, and rare target reagents, helping you bridge from discovery to diagnostic development seamlessly.