Anti-HINT1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49773

Core Product Specifications and Parameters

Biological Background: HINT1 Function and Localization

• HINT1 is a member of the histidine triad (HIT) nucleotide-binding protein family, also known as protein kinase C inhibitor 1 (PKCI-1) and adenosine 5'-monophosphoramidase.
• It exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing a range of purine nucleotide phosphoramidates including AMP-NH2, AMP-morpholidate, GMP-morpholidate, and aminoacyl-AMP conjugates such as lysyl-AMP (PubMed:15703176, 16835243, 17217311, 17337452, 22329685). Related references: PMID:15703176 PMID:16835243 PMID:17217311 PMID:17337452 PMID:22329685
• HINT1 also hydrolyzes 3-indolepropionic acyl-adenylate and tryptamine adenosine phosphoramidate monoester and other fluorogenic substrates in vitro (PubMed:17217311, 17337452, 23614568). Related references: PMID:17217311 PMID:17337452 PMID:23614568
• It converts adenosine/guanosine 5'-O-phosphorothioate to nucleoside 5'-O-phosphates with concurrent hydrogen sulfide release (PubMed:30772266). Related references: PMID:30772266
• As a scaffolding protein, HINT1 modulates transcriptional activation by the LEF1/TCF1-CTNNB1 and MITF-CTNNB1 complexes (PubMed:16014379, 22647378). Related references: PMID:16014379 PMID:22647378
• HINT1 modulates p53/TP53 levels and p53-mediated apoptosis (PubMed:16835243). Related references: PMID:16835243
• It modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box) E3 ubiquitin-protein ligase complex (PubMed:19112177). Related references: PMID:19112177
• HINT1 displays SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17 (PubMed:31088288) and RANGAP1 (by similarity). Related references: PMID:31088288
• Localized in both cytoplasm and nucleus, HINT1 is widely expressed across tissues and is subject to acetylation and phosphorylation; it is implicated in neuropathy and cancer-related pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human HINT1, suggesting the antibody detects the N-terminal region; consider this for isoform specificity.
• For Western blotting, a band at approximately 14 kDa is expected; validate using appropriate positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and blocking conditions for your tissue/cell type.
• The antibody cross-reacts with mouse and rat HINT1, supporting translational and comparative studies; verify reactivity in your sample system.
• For ELISA applications, titrate the antibody and use validated standards to ensure assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies like this anti-HINT1 rabbit mAb, and bulk ancillary reagents to support nucleotide metabolism and transcription regulation research. From initial concept to clinical application, CamelBio’s one-stop sourcing and technical consulting services help accelerate your diagnostic development with reliable supply and expert guidance.