Anti-HILPDA Polyclonal Antibody for IHC-P, ELISA - Q9Y5L2

Core Product Specifications and Parameters

Biological Background: HILPDA Function and Localization

• HILPDA (hypoxia-inducible lipid droplet-associated protein), also known as HIG2, HIG-2, and C7orf68, is a 63-amino acid protein encoded by the human gene HILPDA.
• It functions to increase intracellular lipid accumulation and promotes lipid droplet formation.
• HILPDA stimulates the expression of cytokines such as IL6, MIF, and VEGFA, linking it to inflammatory and angiogenic pathways.
• The protein enhances cell growth and proliferation, potentially contributing to tumor progression.
• Subcellularly, HILPDA localizes to lipid droplets, can be secreted, and associates with membranes, consistent with its transmembrane domain and role in lipid metabolism.
• Expression is highly upregulated in renal cell carcinoma and detected in cervical and endometrial cancers, with minimal expression in normal adult tissues; fetal kidney also shows expression.
• Post-translational modifications include phosphorylation, and the protein is classified as a stress response factor with a transmembrane helix.
• Note: Predicted molecular weight is 7kDa (6950 Da), though migration may vary due to the small size and potential modifications.

Experimental Guidance and Technical Tips

• The immunogen covers the full 1–63 amino acid sequence of human HILPDA, so the antibody may recognize epitopes throughout the N-terminal portion; consider this for detecting truncated or homologous proteins.
• For IHC-P on tissue sections, standard antigen retrieval and permeabilization protocols optimized for lipid droplet-associated proteins may improve staining, especially in cancer samples.
• The antibody’s reported cross-reactivity with mouse and rat suggests utility in orthologous model systems; however, each species should be validated independently.
• In ELISA applications, use recombinant HILPDA protein as a positive control; note that endogenous HILPDA may be secreted, so cell culture supernatants could be analyzed.
• Due to HILPDA’s small size and membrane association, sample preparation (e.g., lysis buffer choice, sonication) may influence detection; optimize empirically for your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD manufacturers and researchers by consolidating high-quality raw materials—from validated polyclonal antibodies like this HILPDA reagent to bulk ancillary reagents. For hypoxia and cancer biomarker development, our platform offers a full range of antibodies, optimized pairs for assay development, and sourcing capabilities for rare targets across human, mouse, and rat species, all supported by our technical consulting services to accelerate your project from concept to clinic.