Anti-HIGD1A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y241

Core Product Specifications and Parameters

Biological Background: HIGD1A Function and Localization

• HIGD1A (HIG1 domain family member 1A) is also known as Hypoxia-inducible gene 1 protein and RCF1 homolog A (RCF1a).
• It is proposed to function as a subunit of cytochrome c oxidase (COX, complex IV), the terminal enzyme of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water.
• It may play a role in the assembly of respiratory supercomplexes, which facilitate efficient electron transport.
• Subcellularly, HIGD1A localizes to the mitochondrion membrane and mitochondrion inner membrane.
• Post‑translational modifications include acetylation and phosphorylation, as identified by proteomics approaches.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• The protein is associated with the cellular stress response, linking it to its hypoxia‑inducible nature.

Experimental Guidance and Technical Tips

• For western blot (WB), expect a band near 10 kDa; consider using whole‑cell lysates from human cell lines as positive controls.
• In immunohistochemistry (IHC‑P), antigen retrieval conditions (e.g., heat‑induced epitope retrieval in citrate buffer) may need optimization.
• For immunofluorescence (IF/ICC), include appropriate controls to confirm mitochondrial localization.
• As a polyclonal antibody, ensure consistency across batches by reserving aliquots of the same lot.
• ELISA performance can be assessed using the immunizing peptide as a reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one‑stop sourcing solution for IVD raw materials, connecting diagnostic manufacturers and research teams with high‑quality reagents. For mitochondrial research targets like HIGD1A, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Should you need difficult‑to‑source raw materials or custom production, our team is ready to support your project from concept to clinic.