Anti-HIF3A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2N7

Core Product Specifications and Parameters

Biological Background: HIF3A Function and Localization

• HIF3A encodes hypoxia-inducible factor 3-alpha, a basic-helix-loop-helix-PAS (bHLH-PAS) transcription factor also known as IPAS, MOP7, PASD7, bHLHe17, and HIF3-alpha-1.
• It acts as a transcriptional regulator in adaptive response to low oxygen tension, attenuating the ability of HIF1A and EPAS1/HIF2A to bind to hypoxia-responsive elements (HRE) and inhibiting HRE-driven transcriptional activation.
  Related references:
  PMID:11573933, PMID:16126907, PMID:17998805, PMID:19694616
• HIF3A functions as an inhibitor of angiogenesis in hypoxic corneal cells and may act as a tumor suppressor, inhibiting malignant cell transformation.
  Related references:
  PMID:17998805
• It plays a role in the development of the cardiorespiratory system and may be involved in apoptosis (By similarity).
• The protein localizes to the nucleus, cytoplasm, nucleus speckle, and mitochondrion.
• HIF3A is expressed in vascular cells, kidney, lung epithelial cells, endothelial cells, vascular smooth muscle cells, heart, placenta, and skeletal muscle, with weaker expression in lung, liver, and kidney. Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle, and pancreas, while isoforms 3 and 4 show weak fetal expression.
• Keywords associated with HIF3A include alternative splicing, angiogenesis, apoptosis, cytoplasm, developmental protein, hydroxylation, isopeptide bond, mitochondrion, nucleus, proteomics identification, reference proteome, repeat, repressor, stress response, transcription, transcription regulation, tumor suppressor, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-130) of human HIF3A; consider verifying isoform specificity in your experimental system.
• For Western blot (WB), the predicted molecular weight is 72 kDa. Validate with positive and negative controls from relevant tissues (e.g., kidney, heart) under normoxic and hypoxic conditions.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions as HIF3A can localize to multiple subcellular compartments.
• For ELISA, ensure the antibody is suitable for your specific assay format; consider using recombinant HIF3A protein as a standard.
• Cross-reactivity is reported for human, mouse, and rat; for other species, sequence alignment is recommended to predict reactivity.
• Since HIF3A expression is regulated by oxygen levels, consider inducing hypoxia (e.g., 1% O2 or CoCl2 treatment) in cell culture to upregulate target levels before detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs and recombinant proteins for hypoxia and cancer biomarker development. Our HIF3A polyclonal antibody supports advanced research into oxygen-sensing pathways, tumor angiogenesis, and cardiorespiratory biology. As your integrated sourcing partner, we also supply bulk ancillary reagents and facilitate rare-target raw-material procurement to accelerate your projects from concept to clinic.