Anti-HIF1β/ARNT Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P27540

Core Product Specifications and Parameters

Biological Background: HIF1β/ARNT Function and Localization

• Also known as Aryl hydrocarbon receptor nuclear translocator (ARNT), Class E basic helix-loop-helix protein 2 (bHLHe2), and Hypoxia-inducible factor 1-beta (HIF-1-beta), this protein is encoded by the ARNT gene in humans.
• Serves as an obligate heterodimerization partner for several basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) proteins, enabling transcriptional responses to diverse environmental and developmental signals. Related references: PMID:28396409, PMID:34521881
• Forms heterodimers with the aryl hydrocarbon receptor (AHR) to mediate the transcriptional response to xenobiotics by binding xenobiotic response elements (XREs) or dioxin response elements (DREs) in target gene promoters. Related references: PMID:28396409, PMID:34521881
• Heterodimerizes with hypoxia-inducible factor alpha subunits (HIF1A, EPAS1, NPAS1, or NPAS3) to form hypoxia-inducible transcription factors that bind hypoxia response elements (HREs), activating genes involved in angiogenesis, erythropoiesis, and metabolic adaptation. Related references: PMID:16181639, PMID:20699359
• Plays a central role in environmental sensing and the developmental and physiological regulation of gene expression, integrating signals from oxygen tension and foreign chemicals. Related references: PMID:34521881
• Localizes predominantly to the nucleus, consistent with its function as a transcription factor.
• Subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination (Ubl conjugation), which may regulate its activity, stability, or interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 499–789) targets the C-terminal region of HIF1β, which may confer isoform specificity; verifying reactivity against relevant splice variants is recommended.
• For Western blotting, the predicted molecular weight is ~87 kDa; use an appropriate gel percentage (e.g., 8–10% SDS-PAGE) and validate with positive controls (e.g., nuclear extracts from hypoxic cells).
• In IHC-P, antigen retrieval may be necessary for optimal detection; titrate the antibody on known positive human tissues (e.g., kidney or tumor sections) and include isotype controls.
• Pair with HIF1α antibodies for co-immunoprecipitation or dual-fluorescence studies to investigate hypoxia-induced complex formation.
• This antibody has been reported for ELISA applications; consider its use as a capture or detection reagent after in-house buffer optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HIF1β/ARNT antibody supports research into hypoxic signaling and environmental response pathways. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio is your trusted partner for IVD material procurement.