Anti-HIF1AN/FIH1 Rabbit Polyclonal Antibody [KO Validated] for WB, IHC-P, IF/ICC, IP, ELISA - Q9NWT6

Core Product Specifications and Parameters

Biological Background: HIF1AN Function and Localization

• Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), also known as Factor inhibiting HIF-1 (FIH-1) and hypoxia-inducible factor asparagine hydroxylase, is a dioxygenase enzyme.
• HIF1AN hydroxylates HIF-1 alpha at asparagine-803 in the C-terminal transactivation domain, acting as an oxygen sensor to regulate the hypoxic response.
• Under normoxic conditions, this hydroxylation prevents the interaction of HIF-1 with transcriptional coactivators such as CBP/p300, thereby repressing hypoxia-inducible gene expression.
• Beyond HIF, HIF1AN hydroxylates asparagine, aspartate, and histidine residues within ankyrin repeat domains of diverse substrates (e.g., NFKB1, NOTCH1, ANK1, TNKS2), influencing multiple signaling pathways.
• It negatively regulates NOTCH1 activity, accelerating myogenic differentiation, and positively regulates ASB4 activity to promote vascular differentiation.
• HIF1AN localizes predominantly to the nucleus but is also found in the cytoplasm and perinuclear region.
• Additional keywords include dioxygenase, iron-binding, metal-binding, oxidoreductase, and acetylation, reflecting its catalytic and regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-349, nearly full-length) may generate polyclonal antibodies recognizing multiple epitopes, potentially enhancing detection sensitivity.
• For Western blot, expect a specific band near 41 kDa; knockout (KO) validation is strongly recommended to confirm antibody specificity.
• In IHC-P, optimize antigen retrieval (e.g., citrate buffer pH 6.0) and validate staining in relevant tissue samples.
• For IF/ICC, fix cells with paraformaldehyde and permeabilize with Triton X-100; confirm staining in appropriate positive control cells.
• For IP, use standard Protein A/G agarose beads for rabbit IgG; include an IgG isotype control.
• For ELISA, titrate the antibody as capture or detection reagent and validate in the relevant sample matrix.
• Reported cross-reactivity with mouse and rat suggests conserved epitopes; nonetheless, verify species sequence alignment for your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined approach to procuring high-quality IVD raw materials. The HIF1AN (FIH1) antibody supports research into hypoxia signaling and related pathways. As your bridging partner, we can source validated antibody pairs, bulk ancillary reagents, and even rare-target raw materials to accelerate your assay development—from concept to clinic.