Anti-HIF1AN/FIH1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NWT6

Core Product Specifications and Parameters

Biological Background: HIF1AN/FIH1 Function and Localization

• HIF1AN (hypoxia-inducible factor 1-alpha inhibitor), also called factor inhibiting HIF-1 (FIH-1), is an asparagine hydroxylase that regulates the hypoxia response.
• Under normoxia, it hydroxylates HIF-1α at Asn-803 in the C-terminal transactivation domain, blocking the recruitment of p300/CBP coactivators and thereby repressing hypoxia-inducible gene transcription.
• Acts as a direct oxygen sensor; its hydroxylase activity requires molecular oxygen, iron, and 2-oxoglutarate, linking cellular oxygen tension to HIF-1α stability and activity.
• Beyond HIF-1α, HIF1AN hydroxylates asparagine, aspartate, and histidine residues in ankyrin repeat domains (ARDs) of diverse proteins, including NFKB1, NOTCH1, ASB4, and TNKS2, modulating signaling pathways beyond hypoxia.
• Negatively regulates Notch signaling by hydroxylating NOTCH1, which accelerates myogenic differentiation; conversely, hydroxylation of ASB4 positively promotes vascular differentiation.
• Subcellular distribution includes both the nucleus and cytoplasm, with perinuclear accumulation, reflecting its roles in transcriptional regulation and cytoplasmic protein interactions.
• Classified as a dioxygenase and iron-dependent oxidoreductase; its active site contains a mononuclear iron center essential for catalysis.

Experimental Guidance and Technical Tips

• The immunogen covers virtually the entire HIF1AN protein (residues 1–349), which encompasses the catalytic JmjC domain; this design is expected to generate antibodies recognizing multiple epitopes, aiding detection in denaturing and native assays.
• For Western blot, a band around 40–41 kDa is anticipated; inclusion of a normoxic cell lysate (e.g., HeLa, HEK293) as a positive control and a hypoxia-treated sample to assess HIF-1α hydroxylation-dependent effects may be informative.
• When performing ELISA, consider coating with recombinant HIF1AN or a HIF-1α peptide; a sandwich ELISA paired with a capture antibody specific for a distinct epitope could increase specificity.
• Predicted cross-reactivity with mouse HIF1AN is supported, but performance should be confirmed using the appropriate tissue or cell extracts.
• Optimal antibody dilutions and assay conditions should be empirically determined for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides HIF1AN/FIH1 polyclonal antibodies alongside a full suite of IVD raw materials, including validated antibody pairs, recombinant antigens, and ancillary reagents for hypoxia and oxygen-sensing research. From monoclonal and polyclonal antibodies to bulk-grade buffers, CamelBio streamlines procurement for diagnostic developers. Leverage CamelBio’s global sourcing expertise to obtain rare-target raw materials, accelerating your assay from concept to clinic.