Anti-HIF1AN/FIH-1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NWT6

Core Product Specifications and Parameters

Biological Background: HIF1AN (Factor Inhibiting HIF-1) Function and Localization

• HIF1AN (Factor inhibiting HIF-1), also known as FIH-1 or hypoxia-inducible factor asparagine hydroxylase, is a 40.3 kDa oxygen-dependent dioxygenase encoded by the HIF1AN gene (GeneID 55662).
• It primarily hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD) under normoxic conditions, preventing interaction with transcriptional coactivators like CBP/p300, thereby regulating the hypoxic response.
• In addition to HIF-1α, FIH-1 hydroxylates specific asparagine, aspartate, and histidine residues within ankyrin repeat domains (ARD) of various proteins, including NFKB1, NFKBIA, NOTCH1, ASB4, and TNKS2.
• FIH-1 is predominantly localized in the nucleus and cytoplasm, with enrichment in the perinuclear region, consistent with its dual roles in transcriptional regulation and cytoplasmic signaling.
• Through hydroxylation of NOTCH1, FIH-1 negatively regulates NOTCH1 activity, thereby accelerating myogenic differentiation; conversely, it positively regulates ASB4 to promote vascular differentiation.
• The protein contains iron-binding and dioxygenase catalytic domains, and its activity is modulated by acetylation, highlighting its role as a key sensor linking oxygen availability to transcriptional outcomes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–349, covering the full-length FIH-1 protein; this may elicit polyclonal-like epitope recognition, supporting robust detection in multiple applications. For Western blot, a band around 41 kDa is expected under reducing/denaturing conditions; consider including both normoxic and hypoxic cell lysates to assess endogenous expression.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval protocols may need optimization; control tissues with known high FIH-1 expression (e.g., kidney, liver) should be validated alongside knockout or siRNA-treated samples.
• For ELISA, pairing with a detection antibody against a distinct epitope is advised; ensure standard curves include recombinant HIF1AN protein (full-length or fragment) to confirm assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw materials, specializing in hypoxia and oxygen-sensing research tools. From this recombinant rabbit monoclonal anti-HIF1AN antibody, we extend our offering to validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets like FIH-1 and related epigenetic modifiers. Whether you need optimized monoclonal reagents or technical consultation from concept to clinical application, CamelBio bridges the gap between discovery and diagnostics.