Anti-HIF1α Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16665

Core Product Specifications and Parameters

Biological Background: HIF1α Function and Localization

• HIF1α (hypoxia-inducible factor 1-alpha) is a master transcriptional regulator of the cellular and systemic adaptive response to hypoxia, encoded by the HIF1A gene (also known as BHLHE78, MOP1, PASD8). Related references: PMID:11292861, PMID:11566883, PMID:15465032

• Under hypoxic conditions, HIF1α dimerizes with ARNT and binds to hypoxia response elements (HREs) to activate transcription of over 40 target genes involved in oxygen delivery, angiogenesis, and metabolic reprogramming (e.g., EPO, VEGF, glycolytic enzymes). Related references: PMID:11292861, PMID:11566883, PMID:15465032

• The HIF1α-ARNT heterodimer recruits transcriptional coactivators such as CREBBP and EP300 to initiate target gene expression. Related references: PMID:16543236, PMID:9887100

• It plays a critical role in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic disease. Related references: PMID:22009797

• Subcellular localization is primarily cytoplasmic under normoxia; upon hypoxic stabilization, HIF1α translocates to the nucleus and nuclear speckles to execute its transcriptional functions.

• HIF1α undergoes multiple post-translational modifications, including hydroxylation, acetylation, phosphorylation, S-nitrosylation, and ubiquitination, which tightly regulate its stability and transcriptional activity.

• Expressed in most human tissues with highest levels in kidney and heart, HIF1α is frequently overexpressed in a wide range of cancers and their metastases due to intratumoral hypoxia and oncogenic mutations.

• During SARS-CoV-2 infection, HIF1α is required for induction of glycolysis in monocytes, promoting a pro-inflammatory state and viral replication. Related references: PMID:32697943

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 400–500 of human HIF1α (NP_001521.1), which may contribute to the antibody's cross-reactivity with mouse and rat orthologs.
• For Western blot (WB), HIF1α typically migrates at 93–96 kDa; additional bands corresponding to splice isoforms or modified forms may be observed. Validate in the relevant sample system, preferably using hypoxic stimuli to increase target expression.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of fixation, antigen retrieval, and signal amplification is recommended. Consider including hypoxic tissue samples as positive controls.
• As with all polyclonal antibodies, lot-to-lot consistency should be verified, and pilot testing is advised to confirm suitability for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials, including this anti-HIF1α polyclonal antibody. As your one-stop sourcing bridge, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare-target raw-material procurement—all tailored to hypoxia and oncology research applications. From concept to clinic, CamelBio helps simplify your supply chain so you can focus on developing reliable diagnostic solutions.