Anti-HIF1α Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q16665

Core Product Specifications and Parameters

Biological Background: HIF1α Function and Localization

• HIF1α is a master transcriptional regulator of the adaptive hypoxic response, activating over 40 genes involved in oxygen delivery and metabolic adaptation (erythropoietin, VEGF, glycolytic enzymes, etc.). Related references: PMID:11292861, PMID:11566883, PMID:15465032
• It forms a heterodimer with ARNT and binds to hypoxia response elements (HREs) containing the core sequence 5'-TACGTG-3' in target gene promoters. Transcriptional activation requires recruitment of coactivators such as CREBBP and EP300, and is enhanced by NCOA1/NCOA2 and APEX1. Related references: PMID:16543236, PMID:9887100, PMID:10594042, PMID:10202154
• In addition to its canonical hypoxic roles, HIF1α participates in the axonal distribution of mitochondria in neurons during hypoxia. Related references: PMID:19528298
• Upon SARS-CoV-2 infection, HIF1α is required for induction of glycolysis and pro-inflammatory state in monocytes, enhancing viral replication and expression of cytokines (IL1B, TNF, IL6, interferons). Related references: PMID:32697943
• HIF1α is ubiquitously expressed with highest levels in kidney and heart; it is commonly overexpressed in cancers due to intratumoral hypoxia or oncogenic mutations, and shows elevated expression in pituitary tumors compared to normal pituitary tissue.
• The protein localizes to the cytoplasm, nucleus, and nuclear speckles, reflecting its dynamic shuttling and transcriptional activity.
• Post-translational modifications include hydroxylation, acetylation, phosphorylation, S-nitrosylation, and ubiquitination, which regulate protein stability and transcriptional activity.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide and is expected to recognize total HIF1α protein across human and mouse species. Confirm reactivity in your specific experimental model.
• For Western blot, anticipate bands at approximately 83, 93, or 96 kDa depending on isoform expression and post-translational modifications; use appropriate molecular weight markers and hypoxia-treated positive controls to verify detection.
• In immunofluorescence/ICC, optimize fixation and permeabilization to preserve both cytoplasmic and nuclear localization signals; induction of HIF1α stabilization by hypoxia mimetics or low oxygen incubation is recommended prior to staining.
• For ELISA applications, validate the antibody pair with recombinant HIF1α or hypoxia-treated cell lysates to ensure linear detection range and sensitivity.
• Since HIF1α is rapidly degraded under normoxia, pre-treat cells with proteasome inhibitors (e.g., MG132) or expose to hypoxia to accumulate sufficient target protein for robust detection in all applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated HIF1α monoclonal antibodies, optimized antibody pairs, and ancillary reagents for hypoxia and cancer research. Our platform supports your development pipeline from concept to clinic, offering bulk supply and rare-target sourcing to accelerate your immunoassay and biomarker discovery programs.