Anti-HIBADH Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P31937

Core Product Specifications and Parameters

Biological Background: HIBADH Function and Localization

• HIBADH is a mitochondrial 3-hydroxyisobutyrate dehydrogenase involved in the catabolism of branched-chain amino acids, particularly valine.
• It functions as an NAD-dependent oxidoreductase, catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
• The protein contains a mitochondrial transit peptide, directing it to the mitochondrion, where it localizes.
• Tissue specificity analysis shows expression in skin fibroblasts.
• Post-translational modifications include acetylation, as identified by proteomics.
• The protein has a 3D-structure available, and it belongs to the reference proteome.
• Its enzymatic activity is central to maintaining metabolic homeostasis of branched-chain amino acid derivatives.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 37-336, which may lead to recognition of linear epitopes; consider this when designing experiments.
• For Western blot (WB), a band around 35kDa is expected; validate in the relevant sample system.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC) may require antigen retrieval steps for optimal performance.
• The antibody is suitable for ELISA; consider pairing it with a matched detection antibody if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This HIBADH polyclonal antibody supports research into mitochondrial metabolism and branched-chain amino acid catabolism. Through CamelBio, you can source validated antibody pairs, bulk ancillary reagents, and customized raw materials for rare targets, all with the efficiency of a centralized supply partner.