Anti-HHLA2 Rabbit Polyclonal Antibody for WB, IHC-P and ELISA - Q9UM44

Core Product Specifications and Parameters

Biological Background: HHLA2 Function and Localization

• HHLA2 (also known as B7-H7, B7y, B7-H5) is a member of the B7 family of immune regulatory ligands, characterized by an immunoglobulin domain, disulfide bonds, and glycosylation motifs.
• It functions as a costimulatory molecule on antigen-presenting cells, interacting with TMIGD2 on T-cells to enhance TCR-mediated activation, proliferation, and cytokine production via an AKT-dependent signaling cascade.
• HHLA2 is a single-pass type I transmembrane glycoprotein localized on the cell surface, with a signal peptide, extracellular Ig-like domains, a transmembrane helix, and a cytoplasmic tail.
• It is expressed in a variety of tissues, including colon, kidney, testis, lung, and pancreas, and at lower levels in small intestine, liver, and skeletal muscle. In immune cells, it is highly expressed in B-cells, dendritic cells, and macrophages, but not detected in T-cells.
• The protein undergoes alternative splicing, producing multiple isoforms that may differ in functional properties.
• Key post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for its structural stability and ligand-receptor interactions.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to the extracellular domain (aa 30-200), was designed to generate antibodies that recognize native HHLA2, potentially suitable for detecting surface-expressed protein in IHC-P and total protein in WB.
• For western blot, consider using reducing conditions to resolve disulfide-bonded structures; the predicted molecular weight is ~47 kDa, which may appear higher due to glycosylation.
• Validate antibody specificity in relevant human cell lines or tissues known to express HHLA2 (e.g., colon, kidney), using negative controls (e.g., T-cells) to confirm absence of cross-reactivity.
• In ELISA, the antibody can be used as capture or detection reagent; establish optimal coating and blocking conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined sourcing platform for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For HHLA2-targeted immuno-oncology research, we supply antibodies, recombinant proteins, and technical consultation to accelerate assay development. Partner with CamelBio to simplify your supply chain and advance projects from concept to clinic.