Anti-HGS Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O14964

Core Product Specifications and Parameters

Biological Background: HGS Function and Localization

• HGS (Hepatocyte growth factor-regulated tyrosine kinase substrate), also known as Hrs or protein pp110, is encoded by the HGS gene in humans and belongs to the ESCRT-0 complex.
• It functions in intracellular signal transduction mediated by cytokines and growth factors; when associated with STAM, it suppresses DNA signaling upon IL-2 and GM-CSF stimulation.
• HGS acts as a key regulator of endosomal sorting, recruiting clathrin and concentrating ubiquitinated receptors within clathrin-coated regions for lysosomal degradation.
• As part of the ESCRT-0 complex with STAM, it recognizes ubiquitinated receptors and transfers them to the multivesicular body (MVB) pathway, mediating down-regulation of receptor tyrosine kinases.
• The protein localizes to the cytoplasm, early endosome membrane, and multivesicular body membrane, consistent with its role in vesicular trafficking.
• HGS is ubiquitously expressed in adult and fetal tissues, with higher levels in testis and peripheral blood leukocytes.
• Additional features include zinc-finger domains, metal-binding, and multiple post-translational modifications such as acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 548-777) of human HGS, suggesting potential reactivity with isoforms containing this region. For Western blotting, a band around 86 kDa is expected; consider using reducing conditions and appropriate loading controls.
• For immunofluorescence/ICC, fixation and permeabilization conditions should be optimized, as HGS localizes to endosomal membranes.
• Immunoprecipitation may enrich HGS and its interacting partners; validate with appropriate negative controls.
• ELISA can be used for quantitative detection; cross-reactivity with mouse and rat should be experimentally confirmed in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic development from concept to clinic. This anti-HGS antibody, targeting a key player in endosomal sorting and receptor down-regulation, complements our portfolio of signaling and trafficking-related reagents. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or sourcing for rare targets, CamelBio is your reliable procurement partner.