Anti-HGF Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14210

Core Product Specifications and Parameters

Biological Background: HGF Function and Localization

• Hepatocyte growth factor (HGF), also known as scatter factor (SF) or hepatopoietin-A, is encoded by the HGF gene and plays a central role in liver regeneration and tissue repair.
• HGF is a potent mitogen for mature parenchymal hepatocytes and acts as a growth factor for a broad spectrum of tissues and cell types. Related references: PMID:20624990
• It serves as the activating ligand for the receptor tyrosine kinase MET, promoting receptor dimerization and downstream MAPK signaling. Related references: PMID:15167892, PMID:20977675
• The protein is synthesized as an inactive single-chain precursor and undergoes proteolytic cleavage to generate a biologically active heterodimer composed of an alpha and a beta chain linked by a disulfide bond.
• HGF contains multiple structural domains, including kringle domains and a serine protease homology region; however, the serine protease domain lacks catalytic activity.
• Mutations in HGF are associated with non-syndromic hearing loss (DFNB39), highlighting its importance beyond hepatic function.
• Alternative splicing yields multiple isoforms, and the protein undergoes several post-translational modifications such as glycosylation and formation of pyrrolidone carboxylic acid.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 106–160 of human HGF, a region that is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band around 83 kDa (full-length precursor) is expected; additional bands corresponding to processed forms may also be detected. Consider validating the detection of cleaved forms in your specific sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization conditions should be optimized for the target tissue. The antibody may be suitable for visualizing HGF in liver, placenta, and other expressing tissues.
• Given the polyclonal nature, lot-to-lot consistency should be verified for critical quantitative applications. We recommend titrating the antibody from 1:500 to 1:2000 for initial Western blot experiments.

CamelBio: Your One-Stop Sourcing Bridge

• As a comprehensive IVD raw-material partner, CamelBio supports diagnostic manufacturers and research laboratories in streamlining their supply chain, from validated antibody pairs to bulk ancillary reagents.
• For studies on hepatocyte growth factor and MET signaling, we offer a range of monoclonal and polyclonal antibodies tailored for diverse assay formats, along with expertise in sourcing rare targets and developing customized immunoreagents.
• Whether you require single batches or ongoing supply, CamelBio is your trusted bridge from concept to clinic.