Anti-HFM1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - A2PYH4

Core Product Specifications and Parameters

Biological Background: HFM1 Function and Localization

• HFM1 is a probable ATP-dependent DNA helicase that is required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
• It is also known as DNA 3'-5' helicase HFM1, SEC63 domain-containing protein 1, MER3, POF9, Si-11, and other aliases.
• The protein is preferentially expressed in testis and ovary, consistent with its role in germ cell meiosis.
• HFM1 contains a SEC63 domain and zinc-finger motifs, which are typical of nucleic acid helicases.
• It possesses ATP-binding and helicase activities, as well as isomerase activity, and functions in a metal-dependent manner.
• Disease-associated variants in HFM1 are linked to premature ovarian failure (POF9), highlighting its clinical relevance in female infertility.
• The gene undergoes alternative splicing, potentially yielding isoforms with distinct regulatory or functional properties.
• Additional keywords from UniProt include reference proteome, nucleotide-binding, and hydrolase, emphasizing its conserved and catalytic nature.

Experimental Guidance and Technical Tips

• For Western blotting, use a gel percentage appropriate for high-molecular-weight proteins (~163 kDa) and include positive controls such as testis or ovary lysates.
• In IHC-P, test on formalin-fixed paraffin-embedded tissue sections from testis or ovary, and optimize antigen retrieval conditions for robust staining.
• For ELISA, consider coating with recombinant HFM1 protein or cell lysates, and validate linearity and sensitivity in the relevant sample matrix.
• Given the cross-reactivity with mouse and rat, verify antibody performance in the corresponding species tissues before large-scale experiments.
• The immunogen spans the N-terminal 1-240 amino acids; thus, it may recognize all known isoforms unless alternative splicing removes this region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for meiosis and infertility research, offering validated polyclonal antibodies such as this anti-HFM1 together with tailored antigen pairs, conjugates, and bulk ancillary reagents. As a dedicated sourcing partner, we help diagnostic manufacturers and labs secure rare-target raw materials and optimize assay performance from concept to clinic.