Anti-HFE Polyclonal Antibody for WB, ELISA - Q30201

Core Product Specifications and Parameters

Biological Background: HFE Function and Localization

• HFE is a cell membrane protein primarily involved in iron transport regulation.
• It binds to the transferrin receptor (TFR) and modulates cellular iron uptake by lowering TFR's affinity for iron-bound transferrin.
• The protein is expressed broadly across human tissues, with the notable exception of the brain.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, which are important for its structure and function.
• Mutations in HFE are linked to hereditary hemochromatosis, an iron overload disease.
• The gene undergoes alternative splicing, resulting in multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 80-306, corresponding to a substantial portion of the extracellular domain. This region shares homology with mouse HFE, supporting cross-reactivity, but specificity should be validated in your target sample system.
• For Western blotting, a band near 40 kDa is expected under reducing conditions. Glycosylation may cause a slightly higher apparent molecular weight; consider using deglycosylation enzymes if precise size determination is required.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent. Optimal coating concentrations and pairing conditions should be empirically determined.
• Include positive controls such as HFE-overexpressing cell lysates and negative controls like brain tissue lysates or knockout samples to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-HFE polyclonal antibody supports your iron metabolism and hemochromatosis research, with reliable supply for diagnostic development. Let CamelBio bridge your concept-to-clinic journey with one-stop procurement services.