Anti-HEYL Rabbit Polyclonal Antibody (HEY3, HRT3, bHLHb33) for WB, ELISA - Q9NQ87

Core Product Specifications and Parameters

Biological Background: HEYL Function and Localization

• HEYL (Hairy/enhancer-of-split related with YRPW motif-like protein), also designated HEY3, HRT3, HESR3, and bHLHb33, is a basic helix-loop-helix (bHLH) transcription factor.
• Acts as a transcriptional repressor by binding to the canonical E box sequence 5'-CACGTG-3', thereby regulating gene expression downstream of Notch signaling.
• Functions as an effector of Notch signaling, potentially required for proper cardiovascular development.
• Represses transcription mediated by the cardiac transcriptional activators GATA4 and GATA6.
• Primarily localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Belongs to the HEY family of transcriptional repressors, which are characterized by a bHLH domain and a conserved YRPW motif.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein corresponding to the N-terminal region (aa 1-100) of human HEYL, may generate antibodies recognizing the native protein and possibly other family members; verify specificity.
• For WB, the predicted molecular weight of HEYL is approximately 35 kDa; however, post-translational modifications or isoform variants might affect migration. Include appropriate controls.
• In ELISA, the polyclonal nature of this antibody could be advantageous for capturing the target; consider optimizing coating and detection conditions.
• Cross-reactivity is reported for human and mouse; for other species, validate reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering a wide range of raw materials including validated antibodies, antigens, and ancillary reagents. This HEYL Rabbit pAb supports investigations into Notch signaling and transcriptional regulation, critical areas in developmental biology and disease research. With expertise in rare target sourcing and scalable supply, CamelBio streamlines your transition from concept to clinical application.