Anti-HEY2 Rabbit Polyclonal Antibody for WB, ELISA - Q9UBP5

Core Product Specifications and Parameters

Biological Background: HEY2 Function and Localization

• HEY2 (hairy/enhancer-of-split related with YRPW motif protein 2) is a basic helix-loop-helix (bHLH) transcription factor encoded by the HEY2 gene; its alternative names include CHF1, HRT2, HERP1, HESR2, and gridlock homolog.
• Functions as a downstream effector of Notch signaling and preferentially binds the canonical E box sequence 5'-CACGTG-3', acting as a transcriptional repressor.
• Specifically represses transcription mediated by the cardiac activators GATA4 and GATA6, indicating a key role in cardiac gene regulation.
• Plays an essential role in cardiovascular development, being required for proper heart and vascular morphogenesis.
• Localizes to the nucleus, consistent with its function as a DNA-binding transcription factor.
• Annotated with keywords including developmental protein, DNA-binding, Notch signaling pathway, nucleus, and transcription regulation, reflecting its multifaceted roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), therefore the antibody is expected to recognize full-length HEY2 and possibly isoforms containing this epitope.
• For western blotting, a band near the predicted molecular weight of ~36 kDa should be observed; validate band identity using positive controls (e.g., human, mouse, or rat cell lysates known to express HEY2).
• Since this is a polyclonal antibody, it may exhibit higher sensitivity but also potential batch-to-batch variability; consider using appropriate blocking and optimized dilutions for your specific samples.
• For ELISA applications, the antibody can be used as capture or detection reagent; confirm optimal pairing with the antigen or a matched antibody pair if developing a sandwich assay.
• Always validate performance in the relevant sample system and under your own experimental conditions; cross-reactivity with human, mouse, and rat is indicated, but testing in other species may require additional verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with reliable one-stop access to IVD raw materials. For projects investigating HEY2 and cardiovascular Notch signaling, we supply validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents, ensuring consistent quality from discovery to clinical application. Our sourcing expertise extends to rare targets, helping you accelerate assay development with confidence.