Anti-HEY1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5J3

Core Product Specifications and Parameters

Biological Background: HEY1 Function and Localization

• HEY1 is a basic helix-loop-helix (bHLH) transcriptional repressor that binds preferentially to the canonical E box sequence 5'-CACGTG-3' (PubMed:11095750). Related references: PMID:11095750

• It functions as a downstream effector of Notch signaling, essential for cardiovascular development, including endocardial epithelial-to-mesenchymal transition critical for cardiac valve and septum formation.

• HEY1 represses transcription by interacting with cardiac transcriptional activators GATA4 and GATA6, as well as neuronal bHLH factors ASCL1 and NEUROD4 (PubMed:15485867). Related references: PMID:15485867

• Involved in the regulation of liver cancer cell self-renewal, highlighting a role in oncogenic pathways (PubMed:25985737). Related references: PMID:25985737

• Promotes maintenance of neuronal precursor cells and influences glial versus neuronal fate specification.

• Localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcription factor.

• Expressed during development in somitic mesoderm, central nervous system, kidney, heart, nasal epithelium, and limbs.

• The HEY1 gene undergoes alternative splicing, generating multiple isoforms that may exhibit distinct regulatory properties.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 33 kDa is expected; consider potential isoform variants due to alternative splicing. Validate detection in your specific sample system.

• For immunofluorescence/ICC, anticipate nuclear staining. Use appropriate controls and optimize fixation/permeabilization conditions for your cell type.

• In ELISA, titrate the antibody concentration to determine optimal working dilution. Cross-reactivity with mouse and rat orthologs suggests utility across these species, but sequence alignment should be verified for the immunogen peptide.

• Given the antibody's raised against a peptide within amino acids 114–129, it may recognize both full-length and truncated variants containing this epitope; validation in relevant tissues (e.g., heart, brain) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD raw materials, supporting your cardiovascular and developmental biology research with this high-quality HEY1 antibody. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target antibody sourcing—all tailored to accelerate your assay development from concept to clinic. Partner with CamelBio for reliable, end-to-end procurement.