Anti-Hexokinase II Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52789

Core Product Specifications and Parameters

Biological Background: Hexokinase II Function and Localization

• Hexokinase-2 (HK2), also known as HKII, HXK2, hexokinase type II, hexokinase-B, and muscle form hexokinase, is a member of the hexokinase family and is a 102 kDa protein encoded by the HK2 gene (Gene ID: 3099).
• Catalyzes the phosphorylation of hexoses, such as D-glucose and D-fructose, to hexose 6-phosphates, mediating the first committed step of glycolysis (PubMed:23185017, PubMed:26985301, PubMed:29298880). Related references: PMID:23185017 PMID:26985301 PMID:29298880
• Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space, thereby inhibiting apoptosis (PubMed:18350175). Related references: PMID:18350175
• Predominantly expressed in insulin-responsive tissues such as skeletal muscle.
• Localizes to the mitochondrial outer membrane and the cytosol.
• Post-translational modifications include acetylation; it contains ATP-binding domains and acts as an allosteric enzyme (UniProt keywords: Acetylation, Allosteric enzyme, ATP-binding).

Experimental Guidance and Technical Tips

• The immunogen (recombinant fragment spanning amino acids 1-120) targets the N-terminal region of human Hexokinase II; consider its potential to detect various isoforms or cleavage products.
• For Western blot (WB), the predicted molecular weight is ~102 kDa; optimize gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), include appropriate antigen retrieval and permeabilization steps; validate in tissues with known HK2 expression (e.g., skeletal muscle, heart).
• Owing to cross-reactivity with mouse and rat, the antibody can be applied across human, mouse, and rat samples in WB, IHC, IF, and ELISA; validate in the relevant species system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is your one‑stop sourcing partner for IVD raw materials, streamlining procurement from concept to clinic. This Hexokinase II antibody supports research into glucose metabolism, glycolysis, and apoptosis, making it ideal for metabolic disorder and cancer diagnostic development. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—ensuring seamless integration into your assay workflows.