Anti-HEXIM2 Rabbit Polyclonal Antibody for WB and ELISA - Q96MH2

Core Product Specifications and Parameters

Biological Background: HEXIM2 Function and Localization

• Protein HEXIM2 (Hexamethylene bis-acetamide-inducible protein 2) is a transcriptional regulator that functions as a general RNA polymerase II transcription inhibitor, as supported by functional studies.
• Core component of the 7SK RNP complex: together with 7SK snRNA, it sequesters P-TEFb in an inactive large 7SK snRNP complex, blocking RNA polymerase II phosphorylation and transcription elongation. Related references: PMID:15713661 PMID:15713662
• HEXIM2 is a member of the HEXIM family and exhibits differential expression with HEXIM1.
• It predominantly localizes to the nucleus.
• Ubiquitously expressed across tissues, with higher abundance in testis.
• Post-translational modification: phosphoprotein; contains coiled-coil domains.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment spanning amino acids 147–286 of human HEXIM2. For Western blotting, validate detection of endogenous HEXIM2 near the expected molecular weight (32 kDa per product specification; ~32.4 kDa per UniProt) in your sample system.
• When performing ELISA, use the recombinant immunogen as a positive control and titrate conditions against native protein to confirm reactivity.
• The antibody cross-reacts with human and mouse HEXIM2; treat each species with appropriate controls and validation.
• As a nuclear protein, adequate nuclear enrichment or suitable lysis protocols should be employed for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, providing diagnostic manufacturers, laboratories, and research institutes with a seamless channel from concept to clinic. For transcription regulation and 7SK RNP complex studies, we offer validated antibody pairs, optimized polyclonal antibodies such as this anti-HEXIM2 antibody, bulk ancillary reagents, and rare target raw-material sourcing, all leveraging our one-stop supply capabilities.