Anti-HEXIM1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O94992

Core Product Specifications and Parameters

Biological Background: HEXIM1 Function and Localization

• Protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, or hexamethylene bis-acetamide-inducible protein 1, is encoded by the HEXIM1 gene.
• Functions as a general RNA polymerase II transcription inhibitor as a core component of the 7SK RNP complex, where it cooperates with 7SK snRNA to sequester P-TEFb in an inactive complex, preventing RNA polymerase II phosphorylation and transcriptional elongation. Related references: PMID:12832472 PMID:14580347 PMID:15201869 PMID:15713661
• Regulates the transcriptional activity of NF-kappa-B, ESR1, NR3C1, and CIITA, indicating broader roles in gene expression control. Related references: PMID:15940264 PMID:15941832 PMID:17088550
• Involved in the DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation and activates innate immunity via the cGAS-STING pathway. Related references: PMID:28712728
• Localizes to both the nucleus and cytoplasm, consistent with its roles in transcriptional regulation and RNA binding.
• Ubiquitously expressed with higher levels in placenta and endocrine tissues; differentially expressed relative to HEXIM2.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 210-359 of human HEXIM1; consider this region when assessing antibody specificity in your target species.
• For WB, expect a band near 41 kDa based on the provided protein weight; validate with appropriate positive and negative controls.
• Cross-reactivity with mouse and rat has been noted; verify reactivity in your specific sample systems using tissue lysates or cell extracts.
• For IHC-P, optimize antigen retrieval and antibody dilution; testing on tissue microarrays may help establish optimal conditions.
• For ELISA, use the antibody as a capture or detection reagent after confirming performance in your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, supplying validated polyclonal and monoclonal antibodies for transcription and immunity research. Our portfolio includes bulk ancillary reagents and custom sourcing for rare targets like HEXIM1, ensuring seamless procurement for diagnostic development. From concept to clinic, CamelBio supports your entire workflow.