Anti-HEXIM1 Rabbit Monoclonal Antibody - O94992

Core Product Specifications and Parameters

Biological Background: HEXIM1 Function and Localization

• HEXIM1 (also known as CLP1, EDG1, HIS1, MAQ1) functions as a general RNA polymerase II transcription inhibitor, playing a key role in transcriptional regulation. Related references: PMID:14580347 · PMID:15201869 · PMID:15713661
• It is a core component of the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex, where it cooperates with 7SK snRNA to sequester positive transcription elongation factor b (P-TEFb) in an inactive state, thereby preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Related references: PMID:12832472 · PMID:14580347 · PMID:15201869
• HEXIM1 belongs to a family that includes HEXIM2, and the two proteins are differentially expressed across tissues.
• Subcellular localization is both nuclear and cytoplasmic, consistent with its roles in transcription and signaling.
• HEXIM1 contributes to innate immune defense by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation and subsequent activation of the cGAS-STING pathway in response to DNA virus infection. Related references: PMID:28712728
• It may also modulate the transcriptional activity of NF-kappa-B, ESR1, NR3C1, and CIITA. Related references: PMID:15940264 · PMID:15941832 · PMID:17088550
• The protein is subject to phosphorylation as indicated by proteomics and other evidence.
• Ubiquitously expressed with relatively higher expression in placenta and endocrine tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 255–359 of human HEXIM1. Verify epitope accessibility and antibody specificity in your target model system.
• For western blot, the predicted molecular weight of HEXIM1 is approximately 41 kDa. Use appropriate loading controls and confirm band identity in lysates from relevant species (human, mouse, rat).
• This antibody is applicable to multiple techniques including WB, IHC-P, IF/ICC, IP, and ELISA. Optimize dilution and incubation conditions for each assay, and include appropriate negative controls.
• When working with tissue sections, consider antigen retrieval methods and validate staining specificity using known positive and negative tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide a comprehensive one-stop sourcing solution for IVD raw materials—from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. This anti-HEXIM1 monoclonal antibody supports research in transcriptional regulation, innate immunity, and related signaling pathways. With a commitment to quality and supply continuity, CamelBio helps you bridge the gap from concept to clinic, offering rare‑target sourcing and expert technical consultation along the way.