Anti-HEXA (Beta-hexosaminidase subunit alpha) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P06865

Core Product Specifications and Parameters

Biological Background: HEXA Function and Localization

• HEXA catalyzes the hydrolysis of N-acetyl-D-hexosamine residues from various glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides. Related references: PMID:11707436, PMID:16698036, PMID:8123671
• Beta-hexosaminidase isozymes (A, B, S) exhibit differential activity: isozymes S and A hydrolyze sulfated substrates, whereas isozyme B prefers neutral substrates. Related references: PMID:11707436
• Only isozyme A can degrade GM2 ganglioside in the presence of the GM2 activator protein, a critical reaction for neuronal lipid catabolism. Related references: PMID:8123671, PMID:8672428, PMID:9694901
• HEXA deficiency causes Tay-Sachs disease, an autosomal recessive GM2 gangliosidosis marked by progressive neurodegeneration and early mortality.
• The enzyme localizes to lysosomes, functioning as a glycoprotein hydrolase active as a zymogen precursor processed into the mature form.
• Alternative splicing generates multiple isoforms; the canonical human HEXA protein has a calculated mass of ~60.7 kDa, consistent with the observed 61 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal domain (aa 368-529) of human HEXA, which may aid in detecting full-length or processed forms; validate in relevant sample systems.
• Suitable for Western blot (WB) detection of endogenous HEXA (predicted ~61 kDa) in human, mouse, and rat lysates; consider using reducing conditions and appropriate controls.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), include appropriate fixation/permeabilization protocols and co-localization with lysosomal markers to confirm specificity.
• ELISA applications can be optimized using the recombinant antibody format for antigen capture or detection; cross-reactivity with rodent species allows translational studies.
• As a monoclonal antibody derived from rabbit, it offers high affinity and low background, but always validate in target tissues or cell lines with known HEXA expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HEXA rabbit monoclonal antibody supports research into lysosomal storage disorders such as Tay-Sachs disease and glycosphingolipid metabolism, with validated performance across multiple applications. For your project needs, CamelBio offers a comprehensive portfolio including optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless supply continuity from discovery to commercialization.