Anti-HES1 Rabbit Polyclonal Antibody for WB, ELISA - Q14469

Core Product Specifications and Parameters

Biological Background: HES1 Function and Localization

• Transcription factor HES1 (also known as HHL, HRY, HES-1, bHLHb39) is a member of the basic helix-loop-helix (bHLH) family of transcriptional repressors.
• It functions as a transcriptional repressor by binding to N-box motifs (5'-CACNAG-3') with high affinity and E-box motifs (5'-CANNTG-3') with low affinity, regulating genes that require bHLH proteins for transcription.
• HES1 acts as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1, thereby modulating muscle differentiation.
• It plays a role in the Fanconi anemia (FA) core complex response to DNA cross-link damage, contributing to the stability and nuclear localization of FA core complex proteins and FANCD2 monoubiquitination.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation and DNA damage response.
• Post-translational modifications include ubiquitin-like conjugation (Ubl conjugation), which may affect its stability or activity.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider the predicted molecular weight of approximately 30 kDa, though post-translational modifications may alter migration. Validate detection in relevant cell lines known to express HES1.
• In ELISA, consider using the recombinant fusion protein corresponding to amino acids 151–280 as a positive control or standard to confirm antibody reactivity.
• Given its polyclonal nature, the antibody may recognize multiple isoforms or modified forms; validate specificity in the target species (human, rat, mouse) using appropriate controls.
• For new applications or species beyond human, rat, and mouse, perform independent validation under optimized experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material sourcing, providing a comprehensive portfolio that includes this HES1 polyclonal antibody for transcriptional regulation and developmental biology research. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your R&D and manufacturing workflows. Contact us to explore how our one-stop supply chain solutions can support your project from concept to clinic.