Anti-HES1 Polyclonal Antibody for WB and ELISA - Q14469

Core Product Specifications and Parameters

Biological Background: HES1 Function and Localization

• HES1 (Hairy and Enhancer of Split 1) is a basic helix-loop-helix (bHLH) transcription factor that acts as a transcriptional repressor, binding to N-box (5'-CACNAG-3') and E-box (5'-CANNTG-3') motifs.
• It plays a key role in regulating cell differentiation, particularly as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1.
• HES1 is involved in the DNA damage response, contributing to the stability and nuclear localization of Fanconi anemia (FA) core complex proteins and the monoubiquitination of FANCD2 following DNA cross-link damage.
• The protein is localized to the nucleus, consistent with its function as a DNA-binding transcription factor.
• HES1 is subject to ubiquitin conjugation (Ubl conjugation) and has been structurally characterized (3D-structure), highlighting its regulatory roles.
• Alternative names include HHL, HRY, HES-1, and bHLHb39, reflecting its homology to the Drosophila Hairy and Enhancer of Split proteins.

Experimental Guidance and Technical Tips

• For Western blot, consider using nuclear extracts from human, mouse, or rat cells, and validate with appropriate positive and negative controls. A band around 30kDa is expected.
• For ELISA, the peptide immunogen may be used as a standard for quantification, but validate in the relevant sample system.
• Cross-reactivity with other HES family members should be assessed based on sequence homology within the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for sourcing high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. Our HES1 antibody supports research in developmental biology, Notch signaling, and cancer, with reliable cross-reactivity across human, mouse, and rat. Leverage our comprehensive supply chain to accelerate your diagnostic development from discovery to commercialization.