Anti-HERC3 Rabbit Polyclonal Antibody for WB, ELISA - Q15034

Core Product Specifications and Parameters

Biological Background: HERC3 Function and Localization

• HERC3 (HECT domain and RCC1-like domain-containing protein 3) is a probable E3 ubiquitin-protein ligase, also known as HECT-type E3 ubiquitin transferase HERC3.
• It mediates the transfer of ubiquitin from an E2 conjugating enzyme to target substrates, functioning in the ubiquitin-proteasome pathway.
• The protein contains a HECT domain and RCC1-like repeats, characteristic of the HERC family of ubiquitin ligases.
• It localizes predominantly to the cytoplasm and is associated with cytoplasmic vesicles, suggesting a role in intracellular trafficking or vesicle-related processes.
• Alternative splicing of HERC3 produces multiple transcript variants, which may contribute to functional diversity.
• The protein is classified as a transferase and is involved in ubiquitin conjugation (Ubl conjugation pathway), as indicated by UniProt keywords.
• Its molecular weight is approximately 117 kDa, and it has been identified in large-scale proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (a.a. 1–200) of human HERC3; antibodies may recognize epitopes within this domain. Consider verifying specificity against full-length protein.
• For Western blot (WB) applications, the predicted molecular weight is 117 kDa; use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• The antibody cross-reacts with mouse HERC3, making it suitable for studies in both human and mouse samples. Validate in relevant species and tissue/cell lines.
• Consider ELISA as a screening or titration method; for functional assays (e.g., ubiquitination), additional validation may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a single point of access to high-quality IVD raw materials like the HERC3 Rabbit pAb, essential for studies on ubiquitin-mediated proteolysis and E3 ligase activity. To accelerate your project from concept to clinic, we supply validated antibody pairs, bulk ancillary reagents, and rare-target sourcing—all backed by technical expertise and reliable supply chains.