Anti-HER2/ErbB2 Rabbit Monoclonal Antibody for WB, IHC, IF - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• Receptor tyrosine-protein kinase erbB-2 (also known as HER2, Neu, CD340) is a member of the epidermal growth factor receptor (EGFR) family and functions as an oncogene in various cancers. It lacks a known direct ligand but acts as a coreceptor to form active signaling complexes with other EGFR family members upon neuregulin binding.
• HER2/ErbB2 plays a critical role in regulating cell growth, differentiation, and survival through downstream signaling pathways, including the PI3K/AKT and RAS/RAF/MEK/ERK cascades. It also mediates microtubule stabilization via the MEMO1-RHOA-DIAPH1 pathway, impacting cell polarity and migration.
• The protein localizes predominantly to the cell membrane, but upon activation can translocate to the nucleus, where it functions as a transcription factor. It binds to specific promoter sequences (e.g., TCAAATTC) and regulates genes such as PTGS2/COX-2 and CDKN1A, enhancing rRNA synthesis and cell growth.
• Subcellular distribution includes cell membrane, ruffled membranes, early endosomes, cytoplasm, perinuclear region, and nucleus, reflecting its dynamic trafficking and multifunctional roles.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, which are essential for its stability, dimerization, and kinase activity.
• Tissue specificity: Highly expressed in a variety of epithelial tumor tissues, including primary breast tumors, as well as tumors from the small bowel, esophagus, kidney, and oral cavity, making it a key biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (aa 23-652), enabling detection of full-length HER2/ErbB2. This region is commonly targeted in many diagnostic antibodies and may provide favorable specificity.
• For Western blotting, the expected band is approximately 138 kDa. Use appropriate molecular weight markers and validate with a positive control cell line known to overexpress HER2, such as SK-BR-3 or BT-474.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), antigen retrieval methods (e.g., citrate buffer pH 6.0, heat-induced) may be required. Titrate the antibody to optimize signal-to-noise ratio for your specific tissue and fixation protocol.
• For multiplex IHC (mIHC), verify compatibility with other primary antibodies and detection systems. Due to its robust expression in certain tumors, consider using a dilution series to avoid channel cross-talk.
• This antibody's reactivity has been validated for human samples; cross-reactivity with other species has not been determined and should be experimentally verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs and monoclonal antibodies like this anti-HER2/ErbB2 clone. Whether developing cancer biomarker assays or exploring ERBB2 signaling pathways, CamelBio offers end-to-end procurement solutions—from optimized primary antibodies and ancillary reagents to niche target sourcing—streamlining your journey from concept to clinic.