Anti-Heparanase 1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y251

Core Product Specifications and Parameters

Biological Background: Heparanase Function and Localization

• Heparanase (HPSE, also known as HPA1, HPR1, HSE1, HPSE1) is an endo-β-glucuronidase that specifically cleaves heparan sulfate proteoglycans (HSPGs) into smaller fragments, playing a key role in extracellular matrix (ECM) degradation and remodeling.
• The enzyme exhibits pH-dependent activity, being essentially inactive at neutral pH but highly active under acidic conditions, such as those encountered during tumor invasion, inflammation, and wound healing.
• Heparanase is initially synthesized as a 65 kDa precursor that undergoes proteolytic processing to yield a mature, active heterodimer composed of 8 kDa and 50 kDa subunits; the mature enzyme localizes primarily to lysosomes and can be secreted.
• Subcellular fractionation and immunohistochemistry studies show Heparanase at the lysosome membrane, in the nucleus, and as a secreted protein, suggesting versatile roles in both intracellular and extracellular microenvironments.
• Tissue expression is prominent in the placenta and spleen, with lower levels in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver, and various tumor tissues; within hair follicles, it is specifically detected in the inner root sheath layers.
• Beyond its enzymatic function, Heparanase modulates cell adhesion, promotes AKT1/PKB phosphorylation via lipid rafts, upregulates VEGF-mediated angiogenesis through SRC activation, and acts as a procoagulant by enhancing factor X activation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 36–275) corresponds to a region that likely excludes the N-terminal signal peptide, potentially targeting the mature form of Heparanase; consider verifying reactivity with both the pro-form (~65 kDa) and processed subunits (50 kDa and 8 kDa) in your assay conditions.
• For western blotting, prepare lysates at neutral pH and consider running a positive control from placenta or spleen tissue, where HPSE expression is high; a band near 61–65 kDa is expected.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), test the antibody on paraffin-embedded or frozen sections of placenta, spleen, or tumor xenografts, which strongly express Heparanase, and include an isotype control.
• For ELISA applications, use as a capture or detection antibody after confirming pairing compatibility; recombinant Heparanase protein (e.g., the immunogen) can serve as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized procurement of rare-target raw materials. For researchers focused on heparanase biology, extracellular matrix remodeling, and tumor metastasis, CamelBio streamlines access to high-quality anti-Heparanase antibodies and related immunological tools, supporting everything from early-stage development to clinical assay manufacturing.