Anti-Heparanase 1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y251

Core Product Specifications and Parameters

Biological Background: Heparanase 1 Function and Localization

• Encoded by the HPSE gene, Heparanase 1 (HPA1) is an endo-β-glucuronidase that specifically cleaves heparan sulfate proteoglycans (HSPGs) in the extracellular matrix (ECM), playing a critical role in ECM degradation and remodeling.
• Its enzymatic activity is pH-dependent: essentially inactive at neutral pH but active under acidic conditions typical of tumor microenvironments and inflammatory sites, facilitating cell invasion and metastasis.
• Subcellularly, Heparanase 1 is localized to lysosomes, secreted, and also found in the nucleus, reflecting its diverse functional roles.
• Post-translational modifications include N-linked glycosylation, and the protein contains disulfide bonds; it is classified as a calcium- and magnesium-dependent hydrolase involved in cell adhesion and signaling.
• It promotes angiogenesis by upregulating SRC-mediated activation of VEGF and enhances shedding of syndecans, contributing to endothelial invasion.
• Acts as a procoagulant by potentiating tissue factor/factor VIIa-mediated activation of factor X.
• Highly expressed in placenta, spleen, and hair follicles, with moderate expression in lymph nodes, thymus, leukocytes, bone marrow, and endothelial cells, linking it to both physiological and pathological tissue remodeling.
• Alternative splicing yields isoforms; the full-length protein has a signal peptide, is glycosylated, and is processed into active forms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 36-275) covers a substantial portion of the N-terminal region of human Heparanase 1 (pro-form). In WB, consider detecting the precursor at ~61 kDa; processed active forms may also be observed depending on sample preparation.
• For IF/ICC, optimize cell fixation and permeabilization conditions to preserve antigenicity, particularly as Heparanase 1 can localize to the nucleus, cytoplasm, or be secreted.
• In ELISA applications, this polyclonal antibody may serve as a capture or detection reagent; validate dilution and pairing in your specific assay setup.
• Cross-reactivity is reported for human, mouse, and rat, but always confirm species reactivity in your experimental system with appropriate positive and negative controls.
• Since batch-to-batch variability can occur with polyclonal antibodies, consider lot-specific validation for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a wide range of IVD raw materials, including validated antibodies, bulk ancillary reagents, and technical support services. For researchers focusing on extracellular matrix biology, cancer metastasis, or inflammatory pathways, we offer one-stop procurement of Heparanase 1-targeting reagents—from optimized polyclonal and monoclonal antibodies to antigen-matched pairs. Contact us to discuss your sourcing needs and how we can help accelerate your assay development from concept to clinic.