Anti-Hemoglobin subunit alpha (HBA1) Rabbit Polyclonal Antibody for WB and ELISA - P69905

Core Product Specifications and Parameters

Biological Background: Hemoglobin subunit alpha Function and Localization

• Hemoglobin subunit alpha (alpha-globin) is the alpha chain of hemoglobin, a heterotetramer of two alpha and two beta chains, encoded by the HBA1 and HBA2 genes.
• Its primary function is oxygen transport from the lungs to peripheral tissues.
• A derived peptide, hemopressin, acts as an antagonist of the cannabinoid receptor CNR1, blocking its signaling. Related references: PMID:18077343
• It is predominantly expressed in red blood cells.
• The protein undergoes various post-translational modifications including acetylation, glycation, and phosphorylation.
• It contains a heme group with an iron atom essential for oxygen binding, and mutations are associated with hereditary hemolytic anemia.
• The mature protein has a molecular weight of approximately 15.2 kDa (UniProt: 15258 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human HBA1; the antibody may recognize both HBA1 and the nearly identical HBA2 (differ by one amino acid). Consider testing cross-reactivity if isoform distinction is critical.
• For Western blot, the expected band is around 15 kDa. Use reducing conditions as hemoglobin is a heterotetramer.
• For ELISA, validate with recombinant HBA1 or red blood cell lysates.
• Since HBA1 is highly abundant in red blood cells, consider appropriate sample preparation to avoid overloading.
• The antibody is validated in human samples; cross-reactivity with other species is not tested based on provided data. If using other species, validate by sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material partner offering validated antibodies, bulk reagents, and rare target sourcing. This rabbit polyclonal anti-HBA1 antibody supports hemoglobin and red blood cell research. We provide end-to-end support from concept to clinic for diagnostic developers.