Anti-Hemoglobin Subunit Alpha (HBA1) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P69905

Core Product Specifications and Parameters

Biological Background: Hemoglobin Subunit Alpha Function and Localization

• Hemoglobin subunit alpha (alpha-globin) is encoded by the HBA1 and HBA2 genes, producing an identical alpha chain that assembles with beta chains to form HbA, the major adult hemoglobin.
• Its primary function is oxygen transport from the lungs to peripheral tissues, mediated by heme iron binding.
• A bioactive peptide derived from alpha-globin, hemopressin, acts as an antagonist of the cannabinoid receptor CNR1, efficiently blocking its signaling. Related references: PMID:18077343
• The protein is specifically expressed in red blood cells, where it constitutes the dominant cytosolic component.
• Post-translational modifications include acetylation, glycation, and phosphorylation; the protein contains a heme prosthetic group and binds iron.
• Mutations in HBA1 or HBA2 are associated with hereditary hemolytic anemia, such as alpha-thalassemia and erythrocytosis.
• Structurally, alpha-globin is a glycoprotein with a molecular weight of approximately 15.2 kDa, and its three-dimensional structure has been extensively characterized.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1–100), suggesting the antibody may recognize both native and denatured forms, making it suitable for WB and IHC-P. For applications like IF-P, validate whether the epitope is accessible under the fixation/permeabilization conditions used.
• Reported cross-reactivity with mouse and rat indicates a conserved epitope, but antibody affinity may vary; consider pilot testing in your specific model system.
• For ELISA, pair this antibody with a capture or detection antibody against a non-overlapping epitope to avoid competition; optimization of coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive, one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For hemoglobin and oxygen transport research, we provide ready-to-use HBA1 antibodies, support rare-target raw-material sourcing, and assist with technical consulting to help accelerate your assay development from concept to clinic.