Anti-HEMGN Rabbit Polyclonal Antibody for WB, ELISA - Q9BXL5

Core Product Specifications and Parameters

Biological Background: HEMGN (Hemogen) Function and Localization

• HEMGN (Hemogen), also known as EDAG-1 or NDR, is a nuclear protein that regulates the proliferation and differentiation of hematopoietic cells.
• It functions as a negative regulator of differentiation; overexpression blocks TPA-induced megakaryocytic differentiation in K562 leukemia cells.
• HEMGN may inhibit apoptosis through activation of the nuclear factor-kappa B (NF-κB) signaling pathway.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation of hematopoietic development.
• Tissue expression is prominent in hematopoietic tissues such as bone marrow, testis, and thymus; also detected in thyroid, spermatids, and various leukemia/lymphoma cell lines and solid tumors (prostate, ovarian).
• UniProt annotations classify HEMGN as a developmental protein and phosphoprotein, suggesting post-translational modifications may modulate its activity in differentiation and survival.
• The full-length protein has a predicted molecular weight of approximately 55.3 kDa (UniProt: Q9BXL5).

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole-cell lysates from human hematopoietic cell lines (e.g., K562, Jurkat) to detect endogenous HEMGN near 55 kDa.
• The polyclonal antibody was raised against an N-terminal peptide (aa 1-100); verify specificity through siRNA knockdown or peptide competition if cross-reactivity is a concern.
• In ELISA, take advantage of polyclonal sensitivity; optimize coating concentrations and use recombinant HEMGN as a positive control.
• Reported cross-reactivity with mouse allows potential use in murine hematopoietic models; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials and technical support, covering every stage from concept to clinic. This HEMGN polyclonal antibody supports investigations into hematopoietic differentiation, leukemia, and apoptosis pathways. For larger-scale or custom requirements—including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or sourcing of rare-target antibodies—CamelBio offers one-on-one consultation to streamline your procurement.