Anti-Heme Oxygenase 1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P14901

Core Product Specifications and Parameters

Biological Background: Heme Oxygenase 1 Function and Localization

• Heme Oxygenase 1 (HO-1), also referred to as P32 protein, is encoded by the Hmox1 gene.
• HO-1 catalyzes the oxidative cleavage of heme at the alpha-methene bridge, releasing carbon monoxide (CO), biliverdin IXalpha, and free ferrous iron (By similarity).
• The enzyme affords cytoprotection against programmed cell death by catabolizing free heme, which would otherwise sensitize cells to apoptosis (By similarity).
• Subcellularly, HO-1 is anchored to the endoplasmic reticulum membrane via a C-terminal transmembrane helix, orienting its catalytic domain toward the cytosol.
• As a metal-binding oxidoreductase, HO-1 contains essential heme and iron binding sites critical for its enzymatic function.
• HO-1 is a phosphoprotein, and post-translational phosphorylation may modulate its activity, particularly in response to cellular stress.
• Key biological processes associated with HO-1 include heme catabolism, apoptosis regulation, and iron recycling, highlighting its importance in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering residues 1-266 of mouse Hmox1, which corresponds to nearly the full-length protein. This design enhances the likelihood of recognizing native conformations in multiple applications.
• For Western blot (WB), the predicted molecular weight is approximately 33 kDa; however, migration may vary depending on cell type and post-translational modifications. Validate in your specific sample system.
• In immunofluorescence on paraffin-embedded sections (IF-P) and immunohistochemistry on paraffin sections (IHC-P), optimize antigen retrieval steps, as the target is a transmembrane ER protein. Consider using a mild detergent or heat-induced epitope retrieval.
• For ELISA, pair this monoclonal antibody with a suitable detection conjugate, and include appropriate controls to confirm specificity for HO-1 across species.
• Due to cross-reactivity with human, mouse, and rat, this antibody is a versatile tool for comparative studies; always verify reactivity in your tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, offering a wide array of IVD raw materials. This anti-Heme Oxygenase 1 rabbit monoclonal antibody exemplifies the quality reagents we supply, suitable for oxidative stress and apoptosis research. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare targets to streamline your assay development from concept to clinic.