Anti-Heme Oxygenase 1 (HO-1/HMOX1) Rabbit Polyclonal Antibody - P09601

Core Product Specifications and Parameters

Biological Background: Heme Oxygenase 1 Function and Localization

• Also known as HO-1, HSP32, HMOX1D, and bK286B10, the human HMOX1 gene (GeneID: 3162) encodes a 288-amino acid protein with a predicted molecular weight of ~33 kDa (UniProt P09601).
• HO-1 catalyzes the oxidative cleavage of heme at the alpha-methene bridge, releasing biliverdin IXalpha, carbon monoxide (CO), and ferrous iron; this rate-limiting step is fundamental to heme catabolism and iron reutilization (PubMed:11121422, PubMed:19556236, PubMed:7703255). Related references: PMID:11121422 PMID:19556236 PMID:7703255
• By degrading pro‑oxidant free heme, HO‑1 exerts a potent cytoprotective effect against apoptosis and oxidative stress, preventing heme‑sensitized programmed cell death (PubMed:20055707). Related references: PMID:20055707
• During SARS‑CoV‑2 infection, HO‑1 promotes viral ORF3A‑mediated autophagy but is not required for ORF3A‑induced reticulophagy.
• The enzyme is an integral membrane protein of the endoplasmic reticulum, anchored via a single C‑terminal transmembrane helix.
• HO‑1 is inducible; at the protein level, it is expressed at higher levels in renal cancer tissue than in normal tissue, consistent with its role in stress adaptation.
• Classification keywords include oxidoreductase, heme/iron/metal binding, phosphoprotein, and host‑virus interaction.

Experimental Guidance and Technical Tips

• The immunogen spans residues 1–288 of human HO‑1; the polyclonal antibody may recognize both full‑length and truncated forms. Consider verifying specificity with knockout or siRNA‑treated samples.
• In Western blotting, a specific band is expected at ~33 kDa under reducing conditions. Post‑translational modifications (e.g., phosphorylation) may cause slight mobility shifts.
• Validated applications include WB, IHC‑P, IF/ICC, and ELISA with human, mouse, and rat samples. For other species or untested applications, empirical optimization (e.g., antibody dilution, antigen retrieval) is recommended.
• For immunohistochemistry on paraffin sections, antigen retrieval conditions should be optimized for the target tissue and fixation protocol.
• When performing quantitative Western blotting, include a suitable loading control (e.g., GAPDH or β‑actin) and validate linear dynamic range for each sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one‑stop partner for IVD raw materials, supporting diagnostic manufacturers and research labs from early‑stage development through clinical applications. This anti‑HMOX1 antibody aligns with CamelBio’s portfolio of high‑quality, validated antibodies and ancillary reagents for oxidative stress, apoptosis, and heme metabolism studies. Leverage CamelBio’s sourcing expertise to secure optimized antibody pairs, bulk quantities of recombinant proteins, and rare target raw materials tailored to your assay development needs.