Anti-HEL308 Rabbit Polyclonal Antibody for WB and ELISA - Q8TDG4

Core Product Specifications and Parameters

Biological Background: HEL308 Function and Localization

• HEL308, also known as Helicase POLQ-like or Mus308-like helicase, is encoded by the HELQ gene in humans.
• It functions as a single-stranded 3'-5' DNA helicase with key roles in homology-driven double-strand break (DSB) repair, including microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA), and synthesis-dependent strand annealing (SDSA) (PubMed:11751861, PubMed:19995904, PubMed:21398521). Related references: PMID:11751861 PMID:19995904 PMID:21398521
• HEL308 possesses both DNA unwinding and annealing activities, efficiently unwinding substrates with 3' overhangs or D-loops (PubMed:34937945). Related references: PMID:34937945
• It forms a complex with RAD51, which stimulates its DNA helicase activity, while interaction with replication protein A (RPA) inhibits unwinding but promotes DNA strand annealing (PubMed:34937945, PubMed:34316696). Related references: PMID:34937945 PMID:34316696
• HEL308 is involved in displacing RPA from single-stranded DNA to facilitate annealing of complementary sequences (PubMed:34316696, PubMed:34937945). Related references: PMID:34316696 PMID:34937945
• The protein localizes to the nucleus and chromosomes, consistent with its role in DNA repair.
• Key structural features include ATP-binding, DNA-binding, and helicase domains, with alternative splicing producing multiple isoforms.
• Keywords: Alternative splicing, ATP-binding, Chromosome, DNA damage, DNA repair, DNA-binding, Helicase, Hydrolase, Isomerase, Nucleotide-binding, Nucleus.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-270 of human HEL308, corresponding to an N-terminal region. Consider potential detection of isoforms if alternative splicing retains this region.
• For Western blotting, the predicted molecular weight is ~124 kDa. Use appropriate molecular weight markers and verify band migration under your experimental conditions.
• When using this antibody in ELISA, optimize coating antigen and antibody dilutions for your specific assay setup.
• The antibody cross-reacts with human, mouse, and rat samples. Validate with relevant species-specific positive and negative controls to confirm specificity.
• Since HEL308 participates in DNA repair pathways, consider inducing DNA damage in your experimental model to assess changes in protein expression or localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a seamless sourcing experience for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HEL308 antibody supports research in DNA repair, genomic stability, and double-strand break mechanisms. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing for rare targets like HEL308. Let CamelBio be your trusted partner in advancing IVD development with reliable and traceable reagents.