Anti-HECTD1 Rabbit Polyclonal Antibody for WB, ELISA - Q9ULT8

Core Product Specifications and Parameters

Biological Background: HECTD1 Function and Localization

• HECTD1 is an E3 ubiquitin-protein ligase that accepts ubiquitin from E2 enzymes and transfers it to specific protein substrates.
• It mediates 'Lys-63'-linked polyubiquitination of HSP90AA1, affecting its intracellular localization and secretion, which is critical for cranial neural tube closure.
• HECTD1 catalyzes the ubiquitination and proteasomal degradation of ZNF622, a ribosomal 60S subunit assembly factor, thereby promoting hematopoietic stem cell renewal. Related references: PMID:33711283
• The protein contains ankyrin (ANK) repeats and a HECT (Homologous to E6-AP Carboxyl Terminus) domain, characteristic of this ligase family.
• According to UniProt, HECTD1 is a phosphoprotein, suggesting regulation by phosphorylation.
• It is classified as a transferase and is involved in the ubiquitin conjugation pathway (Ubl conjugation).
• Genetic studies in model organisms indicate a role in neural development; loss of function is associated with neural tube defects.
• The full-length human protein has a calculated molecular weight of approximately 289kDa, consistent with large multi-domain E3 ligases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 2518–2610) of human HECTD1, which may help in specifically detecting the full-length protein.
• For Western blotting, use a low-percentage polyacrylamide gel (e.g., 6–8%) to adequately resolve the high molecular weight target (~289kDa) and validate the antibody with appropriate positive and negative control lysates.
• For ELISA applications, consider using the recombinant immunogen as a coating antigen to optimize assay conditions and check linearity.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified, and blocking conditions optimized for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, supported by technical and consulting services from R&D to clinical application. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents for immunoassay development. For ubiquitin-pathway targets such as HECTD1, we provide reliable supply solutions to accelerate your diagnostic and research programs.