Anti-HEC1/NDC80 Rabbit Monoclonal Antibody for WB, ELISA - O14777

Core Product Specifications and Parameters

Biological Background: HEC1/NDC80 Function and Localization

• HEC1/NDC80 (Kinetochore protein NDC80 homolog) is also known as Highly expressed in cancer protein, Retinoblastoma-associated protein HEC, and kinetochore-associated protein 2.
• Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Related references: PMID:12351790 PMID:14654001 PMID:14699129
• Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Related references: PMID:15548592 PMID:30409912
• The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Related references: PMID:23085020
• Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. Related references: PMID:23891108 PMID:25743205
• Localizes to the nucleus, specifically at the centromere and kinetochore during mitosis.
• Involved in cell cycle, cell division, and mitotic checkpoint regulation; subject to post-translational modifications including phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 543–642) of human HEC1/NDC80; consider using this antibody to detect full‑length endogenous protein.
• For Western blot analysis, enrich mitotic cell lysates to maximize kinetochore protein signals; the predicted molecular weight is approximately 74 kDa.
• For ELISA, optimize coating conditions with peptide or recombinant HEC1/NDC80 protein to ensure sensitive detection.
• Confirmed reactivity with human HEC1/NDC80; cross-reactivity with other species should be validated in the relevant sample system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one‑stop access to IVD raw materials, technical services, and consulting. This anti‑HEC1/NDC80 rabbit monoclonal antibody complements our portfolio of validated antibody pairs, bulk ancillary reagents, and rare‑target sourcing capabilities, facilitating kinetochore biology and cancer‑related assay development from concept to clinic.