Anti-HEC1/NDC80 Polyclonal Antibody for WB, ELISA - O14777

Core Product Specifications and Parameters

Biological Background: HEC1/NDC80 Function and Localization

• HEC1 (Highly Expressed in Cancer protein 1), also known as NDC80, HEC, TID3, or KNTC2, is a kinetochore protein encoded by the NDC80 gene in humans. It is a component of the essential NDC80 complex required for chromosome segregation and spindle checkpoint activity. Related references: PMID:12351790 PMID:14654001 PMID:14699129
• The NDC80 complex is critical for kinetochore integrity and the organization of stable microtubule binding sites in the outer kinetochore plate. Related references: PMID:15548592 PMID:30409912
• HEC1 synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Related references: PMID:23085020
• The protein plays an essential role in chromosome congression and the end-on attachment of kinetochores to spindle microtubules, ensuring proper chromosome alignment during mitosis. Related references: PMID:23891108 PMID:25743205
• HEC1 localizes to the nucleus, specifically at the centromere and kinetochore regions of chromosomes. It is associated with cell cycle, cell division, centromere, chromosome, kinetochore, and mitosis keywords.
• Post-translational modifications include acetylation and phosphorylation, which regulate its function during mitotic progression.
• The full-length protein has a calculated molecular weight of approximately 74 kDa, consistent with the observed band in western blot.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment (aa 343-642) of human HEC1, covering the C-terminal region known for microtubule-binding activity. This design promotes recognition of native protein isoforms.
• For WB, consider using whole cell lysates from human or mouse cell lines (e.g., HeLa, NIH/3T3) to validate detection of the 74 kDa band.
• In ELISA, the antibody may be used as a capture or detection reagent; titrate appropriately for optimal signal-to-noise ratio in your specific assay format.
• Since HEC1 is a key mitotic regulator, synchronize cells at metaphase if stronger signal is needed for co-localization or functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this HEC1/NDC80 polyclonal antibody for kinetochore and mitosis research. We supply validated antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your development workflow. Partner with CamelBio to efficiently source high-quality reagents and accelerate your project from concept to clinic.