Anti-HEATR1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H583

Core Product Specifications and Parameters

Biological Background: HEATR1 Function and Localization

• HEATR1, also known as BAP28 or UTP10, is a HEAT repeat-containing protein essential for ribosome biogenesis.
• It functions as a ribosome biogenesis factor, required for the recruitment of Myc to nucleoli. Related references: PMID:38225354
• HEATR1 is involved in nucleolar processing of pre-18S ribosomal RNA and is required for optimal pre-rRNA transcription by RNA polymerase I. Related references: PMID:17699751
• It is a component of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit, aiding in RNA folding, modifications, cleavage, and quality control. Related references: PMID:34516797
• HEATR1 localizes to the nucleolus within the nucleus, consistent with its role in ribosome assembly.
• Post-translational modifications include acetylation and phosphorylation, and it is identified as a phosphoprotein.
• The protein has a molecular weight of approximately 242 kDa and contains HEAT repeat domains.

Experimental Guidance and Technical Tips

• The immunogen covers N-terminal amino acids 1-120; consider using this region-specific recognition for detecting full-length HEATR1 in Western blot (predicted molecular weight ~242 kDa).
• For ELISA, validate coating and detection conditions in your sample matrix; the polyclonal nature may offer high sensitivity but requires careful blocking.
• Cross-reactivity is reported for human; verify performance in your specific experimental model (e.g., human cell lines).
• Include appropriate loading and positive controls (e.g., nucleolar extracts) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and research laboratories with high-quality antibodies like this anti-HEATR1 polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your ribosome biogenesis research and assay development from concept to clinic.